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Effect of acidic oligosaccharide sugar chain on scopolamine-induced memory impairment in rats and its related mechanisms. Our previous studies have demonstrated that glyceollins, a family of phytoalexins derived from soybeans, exhibited strong antioxidant activity, both in vitro and in vivo [ 2 , 3 ]. Cells were further incubated in the absence or presence of sample for another 12 h. Nrf2-mediated induction of phase 2 detoxifying enzymes by glyceollins derived from soybean exposed to Aspergillus sojae. Furthermore, the inhibition of heme oxygenase-1 HO-1 , a major downstream enzyme of Nrf2, abolished the suppressive effect of glyceollins against glutamate-induced ROS production and cytotoxicity, confirming that activation of HO-1 by glyceollins is responsible for the neuroprotection. The expression of Hmox1 , which is a gene downstream of the Nrf2 signaling pathway, was also induced by glyceollins in a dose-dependent manner Figure 4 B. Thereafter, the medium was removed and replaced by DMEM containing various concentrations of glyceollins in the presence of 5 mM glutamate. Effect of glyceollins on cognitive function in Nrf2 knockout mice. Published online Jan

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